8:30 Registration and Welcome Coffee
9:30 Opening and Welcome
9:45 Inaugural talk - Perspectives on the impact and future of metaproteomics for microbiome research
Hettich Robert - Oak Ridge National Laboratory, USA
Session 1: Metaproteomics in Health and Disease
Chair: Uzzau Sergio
10:30 "Fecal metaproteomics enables functional characterization of remission in patients with inflammatory bowel disease" Wolf Maximilian - University of Bielefeld, Germany
10:45 "Ultra-fast metaproteomics profiling of type-2-diabetes oral microbiome using PASEF DIA" Samodova Diana - University of Copenhagen, Denmark
11:00 "Evaluation of the effect of 93 chemicals on the Simplified Human Gut Microbiota Model (SIHUMIx) using a high-throughput in vitro screening and metaproteomics" Castañeda-Monsalve Victor - Helmholtz Centre for Environmental Research, Germany
11:15 "Microbiome-derived metabolites as biomarkers for anal cancer screening" Saenz Johan - University of Hohenheim, Germany
11:30 "Getting more insights in the respiratory microbiota of cystic fibrosis patients" Hachemi Hamid - CEA - ProGénoMix Platform, France
11:45 "Metaproteomics and 16S rRNA analysis for Study of Gut Microbiota Establishment in Infants with Cystic Fibrosis" Monteoliva Lucia - Universidad Complutense de Madrid, Spain
12:00 "Market outlook for metaproteomics, a feedback from Phylogene" Metton Isabelle - Phylogene, France
12:30 - 14:00 LUNCH BREAK
14:00 Digging out the major plant-microbe interactions occurring in soil
Keynote - Lidbury Ian - University of Sheffield, England
Session 2: Environmental Metaproteomics
Chair: Matallana-Surget Sabine and Heldal Hagen Live
14:45 "What do we gain from scaled-down environmental metaproteomes? An example in a critical marine microbiome" Held Noelle - ETH Zurich, Switzerland
15:00 "Data Independent Acquisition Metaproteomics of a Natural Marine System Reveal Peptide Predictors of Algal Blooms" Timmins-Schiffman Emma - University of Washington, USA
15:15 "Effect of diet on the gut microbiota of the freshwater sentinel Gammarus fossarum " Dumas Thibaut - CEA - ProGénoMix Platform, France
15:30 "Dynamics of hyperhalophilic microbial communities submitted to long-term cyclic disturbances: a metagenomic and metaproteomic case study." Bosch Rafael - Universitat de les Illes Balears (UIB), Spain
15:40 - 16:10 COFFEE BREAK
16:10 "Polysaccharide-degrading potential of microbiomes in agricultural field denitrification beds" Arntzen Magnus - Norwegian University of Life Sciences, Norway
16:30 "The power of teamwork - Investigating the nutritional cycle of a symbiotic microbial community with tandem mass spectrometry" D'Angelo Grace - Max Planck Institute of Marine Microbiology, Germany
16:40 "Unraveling Functionality and Taxonomic Shifts of a Hydrocarbon-Degrading Bacterial Consortia using Metaproteomics" Al Haid Ali - University of Sheffield, England
16:50 "Evaluating the benefit of dia-PASEF approaches and sample-specific database strategies for metaproteomics of very complex microbiomes" Schmit, Pierre-Olivier - Bruker, France
17:05 Poster session
DAY-2 Wednesday, 26th April 2023
09:00 Host-Microbiome Interactions in Early Life: Focus on Gut Fungi
Keynote - Pettersen Veronika K - UiT The Artic University of Norway, Norway
Session 3: New Methods in Metaproteomics
Chair: Seifert Jana
09:45 "Expanding the Characterization of Microbial Ecosystems using DIA-PASEF Metaproteomics" Gomez Varela David - University of Vienna, Austria
10:05 "Optimized gene annotation for accurate protein sequence database construction in metaproteomics and data-independent acquisition metaproteomics" Qiao Liang - Fudan University, China
10:20 "The first ground truth dataset for the assessment of differential expression analyses in metaproteomics and more" Kunath Benoit - University of Luxembourg, Luxembourg
10:35 "Investigating rumen microbiome functions through seaweed-based nutritional manipulation" Ferrillo Alessandra - Norwegian University of Life Sciences, Norway
10:45 - 11:15 COFFEE BREAK
11:15 "Revealing Protein-Level Functional Redundancy in the Human Gut Microbiome using Ultra-deep Metaproteomics" Li Leyuan - University of Ottawa, Canada
11:30 "Unravelling metabolic heterogeneity and cross-feeding using metaproteomics and stable isotope labelling" Kamrad Stephan - University of Cambridge, England
11:45 "Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry" Hellwig Patrick - Otto von Guericke University Magdeburg, Germany
12:00 "Constructing a peptide library for faster human gut metaproteomics research" Sun Zhongzhi - University of Ottawa, Canada
12:10 "MetaLab-MAG: A Metaproteomic Data Analysis Platform for Genome-Level Characterization of Microbiomes from the Metagenome-Assembled Genomes Database" Cheng Kai - University of Ottawa, Canada
12:20 "Comparison of methods for processing fecal protein extracts on a multiwell plate for metaproteomic analysis" Deledda Maria Antonietta - University of Sassari, Italy
12:30 - 14:00 LUNCH BREAK
"CAMPIs" Session
14:00 “CAMPIs: vision and objectives” Hettich Robert- Oak Ridge National Laboratory, USA
14:10 “CAMPI2: Sample preservation” Uzzau Sergio - University of Sassari, Italy
14:30 “CAMPI3: Extracting functional information” Jagtap Pratik - University of Minnesota, USA
14:50 General discussion: “Improving methodologies & next CAMPI editions”
“Grand Challenge” Session
15:20 “Grand challenge: a prospectus” Figeys Daniel - University of Ottawa, Canada
15:50 Round Table: “Opportunities for the community” Figeys Daniel , Armengaud Jean,
Hettich Robert
16:30 COFFEE BREAK
17:00 EuPA Metaproteomics Initiative Session
17:00 "EuPA MetaP Initiative: Structuring the community" Van den Bossche Tim - Ghent University, Belgium
Kunath Benoit - University of Luxembourg, Luxembourg
20:30 IMS Dinner
DAY-3 Thursday, 27th April 2023
Session 4: Integrative Multiomics and Bioinformatics
Chair: Muth Thilo and Mesuere Bart
09:00 "An approach to integrate metagenomics, metatranscriptomics and metaproteomics data found in public resources" Vizcaino Juan Antonio - EMBL-EBI, England
09:20 "The Peptonizer2000: Probability based taxonomic profiling of microbiome samples using PepGM and Unipept" Holstein Tanja - Bundesanstalt für Materialforschung - und Prüfung, Germany
09:35 "Mistle: Metaproteomic index and spectral library search engine" Nowatzky Yannek - Bundesanstalt für Materialforschung - und Prüfung, Germany
09:50 "Accessible metaproteomic database construction using iterative homology searching with CHEW" Kleikamp Hugo - Aalborg University, Denmark
10:05 "Integrative meta-omics in Galaxy and beyond" Schiml Valerie - Norwegian University of Life Sciences, Norway
10:20 "Unipept Desktop 2.0: construction of targeted reference protein databases for proteogenomics analyses" Verschaffelt Pieter - Ghent University, Belgium
10:35 - 11:05 COFFEE BREAK
11:05 "Bioinformatic Workflow for Metaproteomic Analysis of Host-Microbe Dynamics in Clinical Samples" Jagtap Pratik - University of Minnesota, USA
11:20 "PathwayPilot: A User-Friendly Tool for Visualizing Metabolic Pathways and Navigating the KEGG Database" Vande Moortele Tibo - Ghent University, Belgium
11:30 Round Table – Metadata standards and Repository
Van den Bossche Tim, Heyer Robert, Vizcaino Juan-Antonio
12:30 - 14:00 LUNCH BREAK
Session 5: Trends in Metaproteomics
Chair: Jehmlich Nico
14:00 "Experimental validation that human microbiome phages use alternative genetic coding" Peters Samantha - Oak Ridge National Laboratory, USA
14:15 "Fecal sample collection and room-temperature storage using swabs with preservation medium enables deep characterization of the human gut metaproteome" Tanca Alessandro - University of Sassari, Italy
14:30 "A prospectus for tailoring metaproteomics database using MS/MS proteotyping" Armengaud Jean - CEA - ProGénoMix Platform, France
14:45 "Untargeted data-independent acquisition for metaproteomics of complex microbial samples" Suomi Tomi - University of Turku, Finland
14:55 "Exploring precision prebiotics to target the gut microbiome in Alzheimer"s disease" Qin Hongye - University of Ottawa, Canada
15:15 Closing Talk & Perspectives for the field - Metaproteomic applications in clinical research and drug development
Daniel Figeys - University of Ottawa, Canada
16:15 Concluding discussions and perspectives
17:00 End of the Symposium - Visit of the Pope’s Palace