Microbiome research has provided interesting insights into the role of microorganisms in clinical and environmental research. Mass spectrometry based metaproteomics along with nucleic acid based metagenomics and metatranscriptomics has a great potential in unlocking microbiome functions. This microbiome multi-omics or ‘meta-omics’ analysis requires development of analytical workflows and its integration for biological interpretation.
Attendees to this workshop, will be introduced to the basics of metaproteomics analysis using the Galaxy platform and integrative meta-omics workflows for metagenomics (MetaG), metatranscriptomics (MetaT), and metaproteomics (MetaP) analysis. Workshop attendees will be able to use ViMO (Visualizer for Meta-Omics) to a) perform quality control of Metagenome Assembled Genomes (MAGs); b) overview of taxonomy and explore active pathways; c) functional information for protein and mRNA abundance as well as KEGG annotations and pathways on both mRNA and protein level.
Researchers will benefit from understanding of how to analyze meta-omics data; its integration and visualization and other informatics resources available for functional microbiome research.
- 09:00 - 17:00
09:00 - 09:10 Welcome and quick survey
09:10 - 10:10 Introduction to Microbiome Research and Metaproteomics
10:10 - 10:30 Introduction to the meta-omics /microbiome multi-omics
10:30 - 10:50 Coffee Break
10:50 - 11:50 Introduction to the meta-omics /microbiome multi-omics (continued)
11:50 - 14:00 Lunch Break
14:00 - 14:30 Discuss the multi-omics workflow outputs
14:30 - 14:50 Case study - Biogas reactor
14:50 - 15:40 ViMO analysis
15:40 - 16:00 Coffee Break
16:00 - 16:30 Overview of Informatics Platforms available for functional microbiome analysis
16:30 - 16:50 Summary, Q&A and evaluation